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KMID : 0545120100200020415
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Journal of Microbiology and Biotechnology
2010 Volume.20 No. 2 p.415 ~ p.419
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Omics-based analysis of the luxS mutation in a clinical isolate of Escherichia coli O157:H7 in Korea
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Kim Jong-Chul
Yoon Jang-Won
Kim Jong-Bae
Oh Kyung-Hwan
Park Mi-Sun
Lee Bok-Kwon
Cho Seung-Hak
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Abstract
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The purpose of this study was to investigate the relationship between the global regulatory mechanism known as quorum sensing and expression of virulence factors in Escherichia coli O157:H7. A non-polar luxS deletion was introduced into the chromosome of strain CI03J, a human clinical isolate from South Korea, to create the ΔluxS mutant strain ML03J. Phenotypic characterization of wild-type and mutant strains demonstrated that ML03J had no obvious growth or metabolic defects on 0.2% glucose LB medium, produced a functionally-defective flagellum, and could not utilize sorbose; the biological significance of sorbose utilization is unknown. Omics-based analysis revealed the involvement of LuxS in the transcriptional activation of several flagella/chemotaxis-related genes (flhD; fliA, C, D, S, Z; cheA, Y, and Z), repression of glutamate-dependent acid resistance genes (gadAB), and expression of virulence factors including Shiga toxin, hemolysin, and SepD within the LEE pathogenicity island.
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KEYWORD
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Escherichia coli O157:H7, Clinical isolates:Korea, luxS mutation, Omics
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